| Children |
| | 1. Maria de' Medici, b. 3 Apr 1540, Florence, Firenze, Tuscany, Italy  d. 19 Nov 1557, Florence, Firenze, Tuscany, Italy (Age 17 years) |
| + | 2. Francesco I de' Medici, b. 25 Mar 1541, Florence, Firenze, Tuscany, Italy d. 19 Oct 1587, Medici Villa, Poggio a Caiano, Prato, Tuscany, Italy (Age 46 years) |
| | 3. Isabella de' Medici, b. 31 Aug 1542, Florence, Firenze, Tuscany, Italy d. 16 Jul 1576, Villa Medici, Cerreto Guidi, Florence, Firenze, Tuscany, Italy (Age 33 years) |
| | 4. Giovanni de' Medici, Archbishop of Pisa , b. 28 Sep 1543, Florence, Firenze, Tuscany, Italy d. 20 Nov 1562, Livorno, Livorno, Toscana, Italy (Age 19 years) |
| | 5. Lucrezia de' Medici, b. 7 Jun 1545, Florence, Firenze, Tuscany, Italy d. 21 Apr 1561, Ferrara, Ferrara, Emilia-Romagna, Italy (Age 15 years) |
| | 6. Pietro de' Medici, b. 10 Aug 1546, Florence, Firenze, Tuscany, Italy d. 10 Jun 1547, Florence, Firenze, Tuscany, Italy (Age 0 years) |
| | 7. Garzia de' Medici, b. 5 Jul 1547, Florence, Firenze, Tuscany, Italy d. 6 Dec 1562, Florence, Firenze, Tuscany, Italy (Age 15 years) |
| | 8. Antonio de' Medici, b. 1 Jul 1548, Florence, Firenze, Tuscany, Italy d. Jul 1548, Florence, Firenze, Tuscany, Italy (Age 0 years) |
| | 9. Ferdinando de' Medici, Grand Duke of Tuscany, b. 30 Jul 1549, Florence, Firenze, Tuscany, Italy d. 17 Feb 1609, Florence, Firenze, Tuscany, Italy (Age 59 years) |
| | 10. Anna de' Medici, b. 19 Mar 1553, Florence, Firenze, Tuscany, Italy d. 6 Aug 1553, Florence, Firenze, Tuscany, Italy (Age 0 years) |
| | 11. PIetro de' Medici, b. 3 Jun 1554 d. 25 Apr 1604 (Age 49 years) |
|
1522 - 1562 (40 years)
